The role of invadopodia in glioma invasion and response to therapeutics

Research Opportunity
PhD, Masters by Research, Honours
Number of Honour Places Available
1
Department
Surgery
Location
Royal Melbourne Hospital
Supervisor Email Number Webpage
Dr Stanley Styllianos Stylli sstylli@unimelb.edu.au +61390355236 Personal web page

Project Details

Malignant gliomas caused approximately 2.3% of cancer-related deaths in the USA with over 22,000 new patients expected annually. The most prevalent form of glioma and the tumour with the worst prognosis is the glioblastoma multiforme (GBM,WHO grade IV). The prognosis for patients with GBM tumours remains poor with a median survival of only 14.6 months, after receiving the current standard treatment consisting of surgery, radiotherapy and temozolomide. The characteristic of all gliomas is their extensive infiltration, which thwarts efforts to completely remove or ablate these malignant cells.

A property shared by several types of tumour cells is an ability to form structures known as invadopodia. These are dynamic actin-dependent, membrane protrusions which proteolytically degrade extracellular matrix (ECM) substrates via the activities of numerous proteases. We have observed invadopodia in glioma cell lines and primary tumour cells derived from ex vivo cultured GBM specimens, suggesting a role for invadopodia in glioma invasion.

Invadopodia formation and function are dependent on multiple proteins and signaling pathways. Therefore understanding how invadopodia are regulated and controlled within a tumour cell is essential and strategies aimed at disrupting invadopodia could form the basis of novel anti-invasive therapies for treating glioma patients in the future. This project will involve studies that explore the role of a number of invadopodia proteins in glioma cells, how they contribute to their invasive phenotype and ultimately influence their response to treatment.

Potential Skills/Techniques: Cell Biology techniques including cell culture and cell transfections (overexpression and siRNA gene silencing), western blotting, zymography, immunofluorescence and immunohistochemistry, confocal microscopy, migration/invasion assays, reporter assays, ultracentrifugation, proteomics, RNA Seq, datamining



Faculty Research Themes

Cancer

School Research Themes

Cancer in Medicine



Research Opportunities

PhD, Masters by Research, Honours
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Surgery

Research Group / Unit / Centre

Brain Tumour Biology and Therapies

Research Node

Royal Melbourne Hospital

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