Roles of macrophages subpopulations in tumour microenvironment in gastric cancer

Research Opportunity
PhD students, Masters by Research, Honours students, Master of Biomedical Science
Number of Honour Places Available
2
Number of Master Places Available
1
Department / Centre
Medicine
Location
Royal Melbourne Hospital
Primary Supervisor Email Number Webpage
Dr Adrian Achuthan aaa@unimelb.edu.au 83443298 Personal web page
Co-supervisor Email Number Webpage
Dr Yu-Kuan (Tony) Huang tony.huang@unimelb.edu.au Personal web page
Prof Alex Boussioutas alexb@unimelb.edu.au

Summary Gastric cancer (GC) is the fourth most common cancer globally and 7th in incidence in Australia. It has a poor survival rate which can be attributed to the advanced stage at diagnosis in most patients. The molecular and cellular mechanisms underlying the development of GC are not well described. Tumour-associated macrophages (TAMs) are one of the most abundant immune components in GC. Our recent data demonstrated that the heterogeneity of macrophages within the tumour is present at both micro- and macro-levels due to the gradient change of different macrophage markers, namely IRF8, CD68, CD163 and CD206. This study highlights the need for investigating the roles of macrophage subpopulations in a tissue setting, to identify potential therapeutic candidates and to understand the immune landscape of GC. In this project you will explore the roles of proinflammatory and anti-inflammatory cytokines driving the heterogeneity of macrophage populations and their functions in GC.

Project Details

Project description: Gastric cancer (GC) is the fourth most common cancer globally and 7th in incidence in Australia. It has a poor survival rate which can be attributed to the advanced stage at diagnosis in most patients. The molecular and cellular mechanisms underlying the development of GC are not well described. Tumour-associated macrophages (TAMs) are one of the most abundant immune components in GC. Our recent data demonstrated that the heterogeneity of macrophages within the tumour is present at both micro- and macro-levels due to the gradient change of different macrophage markers, namely IRF8, CD68, CD163 and CD206. This study highlights the need for investigating the roles of macrophage subpopulations in a tissue setting, to identify potential therapeutic candidates and to understand the immune landscape of GC. In this project you will explore the roles of proinflammatory and anti-inflammatory cytokines driving the heterogeneity of macrophage populations and their functions in GC.

Techniques: You will acquire a wide-range of skills in cell biology (primary human monocyte/macrophage culture, ELISA assays and flow cytometry), and biochemistry and molecular biology (Western blotting, Real‐Time PCR and siRNA‐mediated gene knock-down).



Faculty Research Themes

Cancer, Infection and Immunology

School Research Themes

Cancer in Medicine, Cardiometabolic



Research Opportunities

PhD students, Masters by Research, Honours students, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department / Centre

Medicine

Research Node

Royal Melbourne Hospital

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