Multiphoton imaging of induced pluripotent stem cell-derived brainoids
- Research Opportunity
- Honours, Master of Biomedical Science
- Number of Honour Places Available
- Medicine and Radiology
- Florey Institute of Neuroscience & Mental Health
Development of induced pluripotent stem cell (iPSC) approaches has enabled studies of epilepsy mechanisms in patient – based models. This can be achieved by generating 3-D iPSC-derived neuronal cultures, so called brainoids, which present self-organised neuronal assemblies. Video rate imaging of neuronal activity in these millimetre sized assemblies is made possible using short intense pulses of infrared light to image inside the living tissue. Scanning the light rapidly through the tissue causes fluorescence of genetically tagged populations of neurons, expressing Calcium sensitive fluorescent indicators. This allows multiphoton optical mapping of electrical activity in the neural networks.
The brainoid structures will be developed from iPSCs obtained from patients with severe forms of childhood epilepsy. This project combines stem cell biology with novel imaging techniques to aid the understanding of genetic epilepsy mechanisms.
Faculty Research Themes
School Research Themes
Honours, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research
For further information about this research, please contact a supervisor.
Research NodeFlorey Institute of Neuroscience & Mental Health
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