Multiphoton imaging of induced pluripotent stem cell-derived brainoids

Research Opportunity
Honours, Master of Biomedical Science
Number of Honour Places Available
1
Department
Medicine and Radiology
Location
Florey Institute of Neuroscience & Mental Health
Primary Supervisor Email Number Webpage
Dr Snezana Maljevic 0390356392 Personal web page
Co-supervisor Email Number Webpage
Dr Timothy Karle tkarle@unimelb.edu.au

Project Details

Development of induced pluripotent stem cell (iPSC) approaches has enabled studies of epilepsy mechanisms in patient – based models. This can be achieved by generating 3-D iPSC-derived neuronal cultures, so called brainoids, which present self-organised neuronal assemblies. Video rate imaging of neuronal activity in these millimetre sized assemblies is made possible using short intense pulses of infrared light to image inside the living tissue. Scanning the light rapidly through the tissue causes fluorescence of genetically tagged populations of neurons, expressing Calcium sensitive fluorescent indicators. This allows multiphoton optical mapping of electrical activity in the neural networks.

The brainoid structures will be developed from iPSCs obtained from patients with severe forms of childhood epilepsy. This project combines stem cell biology with novel imaging techniques to aid the understanding of genetic epilepsy mechanisms.



Faculty Research Themes

Neuroscience

School Research Themes

Neuroscience & Psychiatry



Research Opportunities

Honours, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Medicine and Radiology

Research Node

Florey Institute of Neuroscience & Mental Health

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