Making B-lymphocytes form pluripotent stem cells

Research Opportunity
PhD students, Masters by Research, Post Doctor Researchers
Department / Centre
Royal Children’s Hospital/Murdoch Childrens Research Institute
Primary Supervisor Email Number Webpage
Professor Ed Stanley Personal web page
Co-supervisor Email Number Webpage
Professor Andrew Elefanty Personal web page

Summary This project involves the generation of B-lymphocytes from pluripotent stem cells.

Project Details

B lymphocytes are a critical arm of the adaptive immune system. The in vitro generation of B cells from human pluripotent stem cells provides an opportunity to study normal immune development, to model disease and to generate cells and products for potential therapeutic use. We have methods for making definitive human hematopoietic lineages from PSCs, enabling us to generate both B and T lymphocyte progenitors in vitro.

This project will involve generating B cells from induced pluripotent stem cells using in vitro and in vivo models. Students will have access to established reagents, such as genetically modified PSC reporter lines, and highly evolved pluripotent stem cell differentiation protocols. Students will learn how to grow, genetically modify and differentiate pluripotent stem cells. The project will also involve techniques such as flow cytometry, immunofluorescence, confocal microscopy, and quantitative PCR. Depending on the direction of the work, generation and analysis of RNAseq data may also be necessary.

The Immune Development Laboratory is physically located at the Murdoch Children’s Research Institute, part of the Melbourne Children’s Campus, which also includes the Royal Children’s Hospital and University of Melbourne: See our website for further information.

Faculty Research Themes

Infection and Immunology

School Research Themes

Child Health in Medicine

Research Opportunities

PhD students, Masters by Research, Post Doctor Researchers
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department / Centre


Research Group / Unit / Centre

Research Node

Royal Children’s Hospital/Murdoch Childrens Research Institute

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