Identification of blood markers of delayed concussion in children

Research Opportunity
Honours
Number of Honour Places Available
1
Department
Paediatrics
Location
Royal Children’s Hospital/Murdoch Childrens Research Institute
Primary Supervisor Email Number Webpage
Associate Professor Vera Ignjatovic verai@unimelb.edu.au 99366520 Personal web page
Co-supervisor Email Number Webpage
Prof Vicki Anderson vicki.anderson@mcri.edu.au Personal web page
Professor Franz Babl franz.babl@rch.org.au

Summary This project aims to utilize the complex proteomics SWATH-MS dataset to identify plasma proteins associated with delayed clinical outcomes post-concussion in children. Validation using ELISA and/or MS approach will be required.

Project Details

Traumatic brain injury (TBI) is defined as "an alteration in brain function, or other evidence of brain pathology, caused by an external force". Mild traumatic brain injury (mTBI) is one of the most common forms of TBI and accounts for one out of every 220 paediatric patients admitted to the emergency department within the United States, totalling approximately 144,000 patients per year on a national level and 18% of all paediatric head injury  patients.    An audit of children who attended the Royal Children's Hospital (RCH), Melbourne, Australia in 2004 for a head injury found that 90% of the patients were classified as a mTBI. While most children improve within one week following a mTBI, there is growing consensus that symptoms can persist for up to one month in approximately 30% of patients, with approximately 40% of individuals remaining symptomatic 2 weeks post-injury. This differs significantly from that of adults, a population which sees most individuals recover within a 2 week period. These prolonged symptoms, which may include headaches, dizziness, difficulties concentrating, irritability, cognitive/emotional impairments, and behavioural difficulties carry the potential to develop into significant cognitive, academic and emotional-behavioural difficulties if left unchecked. The last decade has seen a progressive shift to blood proteome analysis as a means of assisting the determination of clinical diagnosis, prognosis and outcome in the setting of concussion. To date, very few studies have investigated the differential plasma protein expression following paediatric mTBI, and specifically the association of blood proteins with delayed clinical outcomes post-concussion in children.  We have recently utilized the SWATH-MS analysis to assess changes in expression of up to 400 plasma proteins. This allowed for an unbiased discovery of proteins not yet implicated in concussion research.  This project aims to utilize the complex proteomics SWATH-MS dataset to identify plasma proteins associated with delayed clinical outcomes post-concussion in children. Validation using ELISA and/or MS approach will be required.



Faculty Research Themes

Child Health

School Research Themes

Child Health in Medicine



Research Opportunities

Honours
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Paediatrics

Research Node

Royal Children’s Hospital/Murdoch Childrens Research Institute

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