How to detect a T cell’s response to autoantigen?

Research Opportunity
PhD, Honours, Master of Biomedical Science
Number of Honour Places Available
1
Number of Master Places Available
1
Department
Medicine and Radiology
Location
St Vincent's Institute of Medical Research
Primary Supervisor Email Number Webpage
A/Prof Stuart Mannering smannering@svi.edu.au Personal web page
Co-supervisor Email Number Webpage
Dr Colleen Elso celso@svi.edu.au
Dr Pushpak Bhattacharjee pbhattacharjee@svi.edu.au

Summary The goal of the research undertaken in the Mannering Lab is to identify the antigens recognized by human T cells implicated in the immune pathogenesis of type 1 diabetes. We take a functional approach to identifying antigens this means that we identify antigens based on a T cell’s response.

Project Details

The goal of the research undertaken in the Mannering Lab is to identify the antigens recognized by human T cells implicated in the immune pathogenesis of type 1 diabetes. We take a functional approach to identifying antigens this means that we identify antigens based on a T cell’s response. Traditionally this has been done using primary T-cell clones. These are T cells isolated from the blood, or more recently the pancreatic islets, which are grown and characterized in vitro. Unfortunately, primary T cells cannot be grown indefinitely in vitro. To circumvent this problem we have developed T-cell avatars, where the T-cell receptor from a primary T cell is cloned and transduced into a cell line. These avatars, which can be grown indefinitely are then used to analyse the TCR’s specificity. However, we need robust and sensitive reporters that can indicate when a TCR has recognized an antigen.

The aim of this project is to develop a panel of promoter constructs that faithfully report on T-cell activation in a TCR transduced cell line. The student working on this project will develop high level skills in molecular biology, retroviral transduction and T-cell immunology.



Faculty Research Themes

Infection and Immunology

School Research Themes



Research Opportunities

PhD, Honours, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Medicine and Radiology

Research Node

St Vincent's Institute of Medical Research

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