“Glass Brain” 3D brain mapping in health and disease.

Research Opportunity
Honours students, Master of Biomedical Science
Number of Honour Places Available
Number of Master Places Available
Department / Centre
Medicine and Radiology
Florey Institute of Neuroscience & Mental Health
Primary Supervisor Email Number Webpage
Dr Kay Richards kay.richards@florey.edu.au 0390356398
Co-supervisor Email Number Webpage
Dr Tim Karle tkarle@unimelb.edu.au Personal web page
Professor Steven Petrou Steve.petrou@florey.edu.au

Summary This project will be using methods to achieve clear brain tissue and to investigate in 3 dimensions the architecture of the brain in health and disease. Our focus is on brain development using genetic epilepsy and Autism mouse models.

Project Details

Histochemical optically clearing of whole tissue samples and the development of new microscopes that can image deep within the tissue, have created unprecedented insight into the wiring of brain networks. Changes in the wiring of cortical neurons, in particular, have been implicated in a number of disorders such as epilepsy, schizophrenia, autism and depression. In this project the candidate will clear whole brains; allowing imaging of neurons, which have been labelled with fluorescent tags. Multi-photon excitation and custom laser light-sheet based microscopy will allow acquisition and reconstruction of exquisite 3D images in key regions of the mouse cortex. The workflow will include chemical clearing, optical microscopy and  big data sets which will be generated. By comparing normal and epilepsy models this work will begin to unravel the changes that occur prior to and after the occurrence of seizures. This will shed important light on the scale on which structural changes occur in neurological disease and will guide future experimental and clinical work.

Faculty Research Themes


School Research Themes

Neuroscience & Psychiatry

Research Opportunities

Honours students, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department / Centre

Medicine and Radiology

Research Node

Florey Institute of Neuroscience & Mental Health

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