Generation of an ex vivo regeneration model for 3D bioprinting applications

Summary The goal of our research is to prevent the onset of OA by regenerating cartilage using a unique 3D printing technology, that requires multiple iteration steps to select the optimal bioink to efficiently regenerate cartilage.New models for evaluating cartilage repair/regeneration are of great value for transferring various culture systems into clinically relevant situations. The repair process can be better monitored in ex vivo systems than in in vitro cell cultures. The aim of this project is to establish an ex vivo osteochondral model prepared from human articular cartilage harvest.

Project Details

The goal of our research is to prevent the onset of OA by regenerating cartilage using a unique 3D printing technology, that requires multiple iteration steps to select the optimal bioink to efficiently regenerate cartilage.

New models for evaluating cartilage repair/regeneration are of great value for transferring various culture systems into clinically relevant situations. The repair process can be better monitored in ex vivo systems than in in vitro cell cultures. The aim of this project is to establish an ex vivo osteochondral model prepared from human articular cartilage harvest (Figure1). The evaluation of cartilage repair will be obtained by creating a defect into the osteochondral plug. With this model it will be possible to score the efficiency of different 3D bioscaffolds in regenerating cartilage, using stem cells laden in different hydrogel based materials.

This project is suited for a Master/PhD research project.

This project will provide opportunity for the student to take part in a discovery project embedded in a multidisciplinary team based at the 3DBioFab@ACMD, St.Vincent Hospital, Melbourne.

The candidate will acquire expertise in cell biology, 3D bioprinting and mechanical bioengineering.