Engineering a tissue flap

Research Opportunity
PhD, Honours
Number of Honour Places Available
1
Department
Surgery
Location
St Vincent's Institute of Medical Research
Primary Supervisor Email Number Webpage
Dr Geraldine Mitchell gmitchell@svi.edu.au Personal web page
Co-supervisor Email Number Webpage
Dr Anne Kong akong@svi.edu.au

Summary We have assembled pre-vascularized scaffolds in the laboratory, by seeding human induced pluripotent stem cell derived endothelial cells (iPSC ECs) into a porous scaffold, with the formation of an interconnected human capillary network within 24 hours. When implanted in vivo into a wound this pre-vascularized scaffold survives and connects to the host blood circulation. We have also successfully connected this human capillary network to a large artery and vein in an animal model thereby establishing the basis of a tissue flap – large vessels connected to a capillary network. This project will progress our hiPSC flap tissue with the addition of muscle tissue, and or fat tissue and/or skin tissue, largely developed from hiPSC.

Project Details

Providing a functional capillary network connected to the blood circulation is a major hurdle in engineering of tissues and organs. The Vascular Biology Group at the O’Brien Institute/St Vincent’s Institute is researching techniques to improve the vascularization of tissue engineering constructs.

We have assembled pre-vascularized scaffolds in the laboratory, by seeding human induced pluripotent stem cell derived endothelial cells (iPSC ECs) into a porous scaffold, with the formation of an interconnected human capillary network within 24 hours. When implanted in vivo into a wound this pre-vascularized scaffold survives and connects to the host blood circulation. We have also successfully connected this human capillary network to a large artery and vein in an animal model thereby establishing the basis of a tissue flap – large vessels connected to a capillary network.

This project will progress our hiPSC flap tissue with the addition of muscle tissue, and or fat tissue and/or skin tissue, largely developed from hiPSC.

The project will involve differentiation and characterization of these cell types from human iPSC and subsequent assembly of various cell combinations including a capillary network in scaffolds and extra-cellular matrices in the cell culture laboratory.  In vivo testing of the tissues developed will occur post-optimization of 3D tissues in vitro. The project will largely involve 3-dimensional cell culture techniques, immunohistochemistry and microscopy techniques.


School Research Themes

Cardiometabolic



Research Opportunities

PhD, Honours
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Surgery

Research Node

St Vincent's Institute of Medical Research

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