Effect of uraemic toxins of vascular reactivity

Research Opportunity
Honours, Master of Biomedical Science
Number of Honour Places Available
1
Number of Master Places Available
1
Department
Medicine and Radiology
Location
St Vincent's Hospital
Primary Supervisor Email Number Webpage
Dr Andrew Kompa akompa@unimelb.edu.au Personal web page

Summary This project will assess the vascular reactivity of aortic vessels exposed to the uraemic toxin IS and its inhibition using selective AhR antagonists in aortic rings. Following experiments the tissue will be examined using immunohistochemistry and real time PCR.

Project Details

Cardiovascular disease in the setting of chronic kidney disease (CKD) displays unique characteristics, primarily left ventricular (LV) hypertrophy with extensive interstitial fibrosis as well as endothelial dysfunction, arterial stiffness, calcification and inflammation, collectively termed ‘uraemic cardiomyopathy’. Uraemic toxins are elevated in the circulation of patients with CKD. Indoxyl sulphate (IS) is one such uraemic toxin that has been extensively examined in cells and animal models of disease. IS has been demonstrated to exert deleterious effects in cardiac, renal, vascular and immune cells, and in tissues from man and animal models. Recently an intracellular receptor for IS was identified, the aryl hydrocarbon receptor (AhR), which, when activated, mediates numerous biological processes including inflammation, vascular remodeling, and atherosclerosis. This project will assess the vascular reactivity of aortic vessels exposed to the uraemic toxin IS and its inhibition using selective AhR antagonists in aortic rings. Following experiments the tissue will be examined using immunohistochemistry and real time PCR. This project will potentially identify a novel agent to treat vascular and inflammatory changes in patients’ with CKD.


School Research Themes

Cardiometabolic



Research Opportunities

Honours, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Medicine and Radiology

Research Group / Unit / Centre

Biomedical Translational Research Group: Developing Therapies for Cardiovascular Disease

Research Node

St Vincent's Hospital

MDHS Research library
Explore by researcher, school, project or topic.