Development and validation of qPCR RNA testing for E. coli, K. pneumoniae and S. epidermidis

Research Opportunity
Honours, Master of Biomedical Science
Number of Honour Places Available
1
Number of Master Places Available
1
Department
Paediatrics
Location
Royal Children’s Hospital/Murdoch Childrens Research Institute
Primary Supervisor Email Number Webpage
Doctor Amanda Gwee amanda.gwee@rch.org.au 9345 5522 Personal web page
Co-supervisor Email Number Webpage
Professor Nigel Curtis nigel.curtis@rch.org.au 9345 6366 Personal web page

Summary We aim to develop and validate a qPCR RNA test for E. coli, K. pneumoniae and S. epidermidis that will be used to quantify the bacterial load on a small volume of blood. This test will enable us to determine the rate of decline in the bacterial load with current recommended antibiotic doses.

Project Details

Bacteraemia is associated with significant morbidity and mortality in children. The gold standard diagnostic test for isolation of bacteria from blood is a blood culture. However, there are numerous limitations to blood cultures: 1. the sensitivity depends on the volume of blood taken; 2. it takes between 24 to 48 hours to identify the bacteria; 3. the amount of bacteria present cannot be quantified. We aim to develop and validate a qPCR RNA test for E. coli, K. pneumoniae and S. epidermidis that will be used to quantify the bacterial load on a small volume of blood. This test will enable us to determine the rate of decline in the bacterial load with current recommended antibiotic doses. Thus, we will be able to compare effectiveness of antibiotic treatment and whether treatment can be optimised.



Faculty Research Themes

Child Health

School Research Themes

Child Health in Medicine



Research Opportunities

Honours, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Paediatrics

Research Node

Royal Children’s Hospital/Murdoch Childrens Research Institute

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