Circulating microparticles in neurodegenerative diseases
- Research Opportunity
- PhD, Masters by Research, Honours, Master of Biomedical Science
- Number of Honour Places Available
- Medicine and Radiology
- Royal Melbourne Hospital
|Dr Ben J Guemail@example.com||0390356317||Personal web page|
|Prof. James S. Wileyfirstname.lastname@example.org||0383446386||Personal web page|
Emerging evidence has shown that activated platelets in the circulation mediate inflammatory effects through release of extracellular vesicles including exosomes (<100 nm) and microparticles (100-1,000 nm). Microparticles are membrane bound bodies released from multiple cell types and they carry various cargo (RNA, cytokines, chemokines, enzymes) that participate in cell to cell communication. We have found elevated circulating microparticle counts in MS patients, especially large microparticles (>200 nm) positive for MitoTracker Green staining. We also found that both platelets and extracellular microparticles express functional P2X7 receptor (P2X7, an ATP gated ion channel mediating proinflammatory responses and scavenger activity) on their surface. In this study, we will develop and optimise the method to accurately quantitate the different types of circulating microparticles, examine the linkage between circulating large microparticles and relapse of multiple sclerosis (MS), understand how these microparticles are released, what they contain, and the pathogenesis potential. The study will provide novel insight on the pathogenesis of the acute MS relapse and other neurodegenerative diseases involving neuroinflammation. The easy assay for large mitochondria-containing microparticles in human plasma may prove to be a biomarker of the risk of acute relapse of MS, which may aid in the choice of different treatments and help the search for more potent and reliable therapies in MS.
Techniques involved are flow cytometry, ELISA, western blotting and cell culture.
Faculty Research Themes
School Research Themes
PhD, Masters by Research, Honours, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research
For further information about this research, please contact a supervisor.
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