Cancer drug discovery by inhibition of a DNA repair pathway with Crispr/Cas9 gene editing and biochemistry

Research Opportunity
PhD, Masters by Research, Honours, Master of Biomedical Science
Number of Honour Places Available
1
Department
Medicine and Radiology
Location
St Vincent's Institute of Medical Research
Primary Supervisor Email Number Webpage
Dr Wayne Crismani wcrismani@svi.edu.au 03 9231 3251

Project Details

Want to cure cancer? We do too. Join our dynamic young team of experts, in the identification and characterisation of new potential cancer therapeutics. In this project, you would learn about DNA repair, genetic diseases like familial breast cancer, and a variety of laboratory-based techniques (CRISPR/Cas9 gene-editing in breast cancer cell lines, AlphaScreen drug discovery assays, recombinant DNA technology, drug discovery, cell-based chemotherapy response assays, pharmacokinetics, protein purification and in vitro enzyme assays).

A new mechanism being used to kill cancers is synthetic lethality – a combination of deficiencies in the expression of two or more genes leads to cell death, whereas a deficiency in only one of these genes does not (see Kais et al below). Our team is working towards identifying new targeted breast and ovarian cancer treatments, which exploits synthetic lethality. The team has made major breakthroughs in reconstituting the necessary DNA repair reactions in vitro, allowing new approaches to design DNA repair-inhibiting drugs. We already have many candidate drugs and gene-editing projects waiting for a motivated candidate.

Our team has a high Post Doc-to-student ratio so there will be plenty of research expertise and support for your project in the laboratory. By joining us you will gain exposure to basic and translational research that is at the forefront internationally. You will receive training in a molecular biology laboratory with a focus on biochemistry and cell biology, increase your understanding of cancer biology and treatment, and increase your employability particularly in the science sector.

$5,000 PhD top ups and Honours scholarships are available to a limited number of outstanding candidates. Scholarships are awarded on a competitive basis and at the discretion of St Vincent's Institute of Medical Research.

Background reading
1.Van Twest et al 2017. Mechanism of Ubiquitination and Deubiquitination in the Fanconi Anemia Pathway. Molecular Cell
2.Kais et al 2016. FANCD2 Maintains Fork Stability in BRCA1/2-Deficient Tumors and Promotes Alternative End-Joining DNA Repair. Cell Reports



Faculty Research Themes

Cancer

School Research Themes

Cancer in Medicine



Research Opportunities

PhD, Masters by Research, Honours, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Medicine and Radiology

Research Node

St Vincent's Institute of Medical Research

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