Bio-engineering complex next generation human liver organoids

Research Opportunity
PhD, Honours
Number of Honour Places Available
1
Department
Surgery
Location
St Vincent's Institute of Medical Research
Primary Supervisor Email Number Webpage
Dr Geraldine Mitchell gmitchell@svi.edu.au Personal web page
Co-supervisor Email Number Webpage
Dr Kiryu Yap kyap@svi.edu.au

Summary This project will use human induced pluripotent stem cells to differentiate all these human liver cell types, characterize the specific cell types and co-culture them in 3-dimensional organoids. Determining the response of these human liver organoids to certain liver disease conditions is also planned.

Project Details

The Vascular Biology Group has developed novel techniques to generate human liver organoids in vitro. The next step in advancing the complexity of this technology is to incorporate additional key elements of liver micro-architecture. This includes a blood vessel network within the organoid, a functioning bile duct system to facilitate bile excretion from organoids and specific liver connective tissue and immune cell types.  This project will use human induced pluripotent stem cells to differentiate all these human liver cell types, characterize the specific cell types and co-culture them in 3-dimensional organoids. Determining the response of these human liver organoids to certain liver disease conditions is also planned.    Techniques used in the project will include (but are not confined to) cell and organoid culture, immunohistochemistry, PCR and a variety of imaging techniques.


School Research Themes

Cardiometabolic



Research Opportunities

PhD, Honours
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Surgery

Research Node

St Vincent's Institute of Medical Research

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