Antibiotic resistance mechanisms in Mycoplasma

Research Opportunity
Honours, Master of Biomedical Science
Number of Honour Places Available
1
Number of Master Places Available
1
Department
Paediatrics
Location
Royal Children’s Hospital/Murdoch Childrens Research Institute
Primary Supervisor Email Number Webpage
Doctor Gerald Murray gerald.murray@mcri.edu.au 8345 3679 Personal web page
Co-supervisor Email Number Webpage
Professor Suzanne Garland suzanne.garland@thewomens.org.au 8345 3670 Personal web page

Summary The project will use molecular methods including Sanger sequencing, quantitative PCR, and digital PCR, in combination with bacterial culture, to investigate the mutations that contribute to antibiotic resistance, and how these mutations arise. Developments in this area will contribute to our understanding of treatment failure.

Project Details

Antibiotic resistance is a substantial and growing problem. Our laboratory is investigating mechanisms of antibiotic resistance in Mycoplasma genitalium, a common sexually-transmitted bacterial pathogen. Infection with M. genitalium causes urethritis in men and can lead to reproductive complications for women.  M. genitalium has an unusual biology and highly reduced genome, making it susceptible to a small spectrum of antimicrobial reagents. Resistance to first line (macrolide) and second line (fluoroquinolone) treatments is high, and increasing. Dual resistance to both classes of antibiotic has increased, resulting in effectively "untreatable" infections. The project will use molecular methods including Sanger sequencing, quantitative PCR, and digital PCR, in combination with bacterial culture, to investigate the mutations that contribute to antibiotic resistance, and how these mutations arise. Developments in this area will contribute to our understanding of treatment failure. This will lead to the development of the next generation of diagnostic assays that report the presence of antibiotic resistance mutations, thereby allowing treatment to be tailored to the individual. The long term outcome will be improved antimicrobial stewardship.



Faculty Research Themes

Child Health

School Research Themes

Child Health in Medicine



Research Opportunities

Honours, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Paediatrics

Research Node

Royal Children’s Hospital/Murdoch Childrens Research Institute

MDHS Research library
Explore by researcher, school, project or topic.