Host defence functions of the novel inflammatory cytokine IL-36G

Summary You will use gene knockout mice to understand how IL-36G prevent infection by bacterial pathogens. The new knowledge you create will help us to develop better ways to treat infections caused by antibiotic-resistant bacteria.

Project Details

The oral cavity is a major entry portal for important bacterial pathogens. We recently demonstrated that the epithelial cells which line the oral cavity produce the inflammatory cytokine IL-36G when they detect the presence of bacteria. Importantly, we have shown that IL-36G not only stimulates the production of proteins which possess direct antimicrobial activities, but also cytokines that activate and recruit immune cells (e.g. neutrophils and lymphocytes). In this project you will use gene knockout mice to investigate how IL-36G prevents infection by bacterial pathogens. The project will provide opportunities to develop skills in bacterial cell culture, mouse models of infection, cytokine assays, gene expression analysis, immunofluorescence confocal microscopy, critical thinking, scientific writing, and oral communication.