Pharmacokinetic and pharmacodynamic analysis of fluorescently tagged molecules with different molecular weights in healthy and respiratory diseased mouse models

Research Opportunity
PhD students, Honours students, Master of Biomedical Science
Number of Honour Places Available
1
Number of Master Places Available
1
Primary Supervisor Email Number Webpage
Dr Joe Ciccotosto jcicco@unimelb.edu.au Personal web page
Co-supervisor Email Number Webpage
Dr Andrew Jarnicki
Dr Robert O'Donoghue

Summary The Ciccotosto group researches respiratory diseases are highly prevalent in the human population where lung function is often compromised due to several factors such as viral infections, chemical irritants, fibrosis, or allergic responses or a combination of these. The epithelial cell lining of the lung is the first line barrier that plays are critical role in filtering out toxic molecules from entering the circulation. The respiratory diseases is often associated with epithelial cell injury resulting in a compromised epithelial cell barrier making it more leaky and altering the lung function. In addition, treatment strategies for respiratory diseases include inhalation of drug molecules that target specific cell surface markers and often need to pass through the epithelial cell barrier. This project will utilise Flouro tagged dextran molecules of different molecular weight sizes and establish a robust assay model for monitoring cellular location, distribution and uptake of molecules through the lungs.

Project Details

Aim of this project: To examine lung permeability and cellular uptake to different molecular weight molecules in healthy and respiratory diseased mouse models in preparation for screening therapeutic drug compounds. 
Techniques: The student will learn animal handling, tissue isolation and dissection. Histological skills including tissue sectioning, staining and microscope imaging. Biochemistry skills including ELISA assay and western blotting



Research Opportunities

PhD students, Honours students, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.


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