Investigating the effect of hepatitis B virus splice variants in liver cells and disease progression

Research Opportunity
Honours
Number of Honour Places Available
1
Department
Microbiology and Immunology
Location
Doherty Institute
Primary Supervisor Email Number Webpage
Professor Peter Revill peter.revill@mh.org.au (03) 9342 9604 Personal web page
Co-supervisor Email Number Webpage
Dr Margaret Littlejohn margaret.littlejohn@mh.org.au (03) 9342 9331 Personal web page
Dr Kai Yan Mak kai.mak@mh.org.au

Summary This Honours project will investigate how splice variants affect liver cells following infection of primary and transformed hepatocytes.

Project Details

Hepatitis B virus (HBV) is one of the most important human pathogens, infecting 257 million people worldwide, including 239,000 Australians. We have previously shown that naturally occurring splice variants of HBV are associated with liver cancer, the 5th most prevalent cancer worldwide, and that splice variants are more diverse than previously appreciated. Although the biological impact(s) of splice variants on liver cells is unclear, we have shown that while splice variants production can be abolished by gene mutations, their replication and infection competence are conserved. Building on these observations, this Honours project will investigate how splice variants affect liver cells following infection of primary and transformed hepatocytes. Techniques utilised will include cell culture, real time PCR/digital PCR, southern, northern and western blotting, quantitative serology, RNA sequencing and basic bioinformatics analysis. This project will make a major contribution to our understanding of the role of HBV splice variants in liver cells and disease progression.



Faculty Research Themes

Infection and Immunology

School Research Themes

Infection & Immunity



Research Opportunities

Honours
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Microbiology and Immunology

Research Node

Doherty Institute

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