Investigating mechanisms of protein trafficking to human mitochondria

Research Opportunity
PhD, Masters by Research, Honours
Department
Biochemistry and Molecular Biology
Location
Bio21 Molecular Science and Biotechnology Institute
Primary Supervisor Email Number Webpage
Dr Diana Stojanovski d.stojanovski@unimelb.edu.au 93053197

Project Details

Mitochondria cannot be created de novo and pre-existing mitochondria are used as templates for mitochondrial biogenesis. This genesis requires the ~1500 different mitochondrial proteins to be imported into the organelle via dynamic translocation machines. Our understanding of protein import and import machineries in higher eukaryotes remains poor. Our lab is leading the way in this research area identifying novel research components and mechanisms (see references below).

Our lab mission is to create new knowledge in mitochondrial biology so that we can improve understanding of the inner workings of the most fundamental unit in life, the cell.

This project will encompass a variety of cell and molecular biology techniques, including cell culture, confocal microscopy, Blue-native PAGE electrophoresis, protein chemistry and proteomics.

References:
Molecular Cell (2017)67: 457-470. “AGK, a mitochondrial kinase associated with Senger’s syndrome, is a subunit of the human TIM22 complex.” Kang K., Stroud DA., Baker MJ., De Souza D., Frazier AE., Liem M., Tull D., Mathivanan M., McConville MJ., Thorburn DR., Ryan MT., and Stojanovski D.

eLife (2016) 24: pii: e17463. “Tim29 is a novel subunit of the human TIM22 translocase and is involved in complex assembly and stability.” Kang Y., Baker MJ., Liem M., Louber J., McKenzie M., Atukorala I., Ang CS., Keerthikumar S., Mathivanan S., Stojanovski D.


School Research Themes

Molecular Mechanisms of Disease, Systems Biology



Research Opportunities

PhD, Masters by Research, Honours
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Biochemistry and Molecular Biology

Research Group / Unit / Centre

Stojanovski laboratory: Mitochondrial biogenesis in health and disease

Research Node

Bio21 Molecular Science and Biotechnology Institute

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