Immunoregulatory functions of the MARCH family of ubiquitin ligases

Research Opportunity
PhD, Masters by Research, Honours
Department
Microbiology and Immunology
Location
Doherty Institute
Primary Supervisor Email Number Webpage
Professor Jose Villadangos j.villadangos@unimelb.edu.au (03) 9035 7684 Personal web page
Co-supervisor Email Number Webpage
Dr Justine Mintern

Summary This project will employ biochemical techniques, microscopy, proteomics, and CRISPR-Cas9 technology to characterise the function of the MARCH family; identify novel MARCH substrates; and characterise the machinery involved in ubiquitination by MARCHs.

Project Details

Protein localisation and abundance (proteostasis) are controlled in eukaryotic cells by regulatory pathways, which remain poorly understood. These pathways regulate changes in protein expression or localisation, in response to environmental cues such as the presence of pathogens. Addition of the small protein ubiquitin (Ub) to membrane proteins by the membrane-associated RING-CH (MARCH) family of ligases is an important mechanism of control of membrane immunoreceptors. This project will employ biochemical techniques, microscopy, proteomics, and CRISPR-Cas9 technology to characterise the function of the MARCH family; identify novel MARCH substrates; and characterise the machinery involved in ubiquitination by MARCHs. The MARCHs have also been shown to play an important role in control of infection by HIV and other enveloped viruses. Our goal is to develop novel therapeutic approaches to fight infection based on manipulation of membrane protein ubiquitination.



Faculty Research Themes

Infection and Immunology

School Research Themes

Infection & Immunity



Research Opportunities

PhD, Masters by Research, Honours
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Microbiology and Immunology

Research Group / Unit / Centre

Villadangos laboratory: Antigen presenting cells & molecules that initiate T cell immunity against pathogens and cancer

Research Node

Doherty Institute

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