Immunoregulatory functions of the MARCH family of ubiquitin ligases

Research Opportunity
PhD, Masters by Research, Honours, Master of Biomedical Science
Number of Honour Places Available
2
Department
Biochemistry and Molecular Biology
Location
Bio21 Molecular Science and Biotechnology Institute
Primary Supervisor Email Number Webpage
Professor Jose Villadangos j.villadangos@unimelb.edu.au Personal web page
Co-supervisor Email Number Webpage
Dr Justine Mintern jmintern@unimelb.edu.au

Project Details

Cell viability and function requires maintenance of the correct protein composition and distribution within the cell at all times. This proteostasis is regulated by mechanisms that control protein synthesis, localisation and degradation. Cells can also respond to external signals to alter proteostasis, allowing them to adapt to changes in their environment.   In cells of the immune system, expression of many important receptors is regulated by addition of the protein ubiquitin by the MARCH family of ligases. MARCH ubiquitination thus plays major roles in immunity against pathogens and cancer. The MARCHs also play important roles in control of infection by viruses.  However we know very little about MARCH specificicity and regulation.

This project will employ biochemical techniques, microscopy, proteomics, and CRISPR-Cas9 technology to characterise the function of the MARCH family; identify novel MARCH substrates; and characterise the machinery involved in ubiquitination by MARCHs. Our goal is to develop novel therapeutic approaches to fight infection and cancer  based on manipulation of membrane protein ubiquitination.

Further reading: Young et al (2008) Nat. Immunol. 9: 1244-1252; Moffat et al (2013) Curr. Opin. Immunol; 25: 109-114; Liu et al (2016), J. Exp. Med. 213:1695-1703.



Faculty Research Themes

Cancer, Infection and Immunology

School Research Themes

Cancer in Biomedicine, Infection & Immunity, Molecular Mechanisms of Disease



Research Opportunities

PhD, Masters by Research, Honours, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Biochemistry and Molecular Biology

Research Group / Unit / Centre

Villadangos laboratory: Antigen presenting cells & molecules that initiate T cell immunity against pathogens and cancer

Research Node

Bio21 Molecular Science and Biotechnology Institute

MDHS Research library
Explore by researcher, school, project or topic.