Development and application of soft culturing microenvironment for novel drug discovery

Research Opportunity
Masters by Research, Honours, Master of Biomedical Science
Department
Pharmacology and Therapeutics
Primary Supervisor Email Number Webpage
Professor Alastair Stewart astew@unimelb.edu.au 8344 5675

Project Details

The mechanical properties of microenvironment for cell culturing used in drug assays have major impacts on the testing outcome. Most assays are currently performed on monolayers of cells in rigid plastic dishes. There is growing interest in efforts to make the assay condition more tissue like in order to better predict the pharmacology of the drugs in tissues. We have referred to these new assay systems in an opinion article describing the interdisciplinary pursuit of “Mechano-pharmacology”. In this project, you will develop assays for drug action in 3- dimensional environments of defined physiological stiffness and compare the results to conventional 2D monolayer plastic culture environments. This interdisciplinary project will involve collaborations with Prof Peter Lee in the Department of Biomedical Engineering and Dr David Simpson and Prof Lloyd Hollenberg in the School of Physics. Methods will include immunoassay, real-time quantitative PCR, cell culture and high content screening using plate-based confocal microscopy (Operetta).



Faculty Research Themes

Infection and Immunology



Research Opportunities

Masters by Research, Honours, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Pharmacology and Therapeutics

Research Group / Unit / Centre

Stewart Laboratory: Mechanopharmacology


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