Characterisation of the changes of the endocytic and secretory pathway of dendritic cells upon activation

Research Opportunity
Masters by Research, Honours, Master of Biomedical Science
Number of Honour Places Available
1
Department
Biochemistry and Molecular Biology
Location
Bio21 Molecular Science and Biotechnology Institute
Supervisor Email Number Webpage
Isabelle Rouiller isabelle.rouiller@unimelb.edu.au 0390354902 Personal web page

Project Details

Dendritic cells are antigen-presenting cells of the mammalian immune system. Their main function is to process antigen material for presentation to T cells. While it is well known that dendritic cells are activated in the process and that activation is associated with the growth of projections (or dendrites), how they process antigen and the associated morphological changes to the endocytic and secretory pathways are far less understood. The endocytic and secretory pathways are essential for antigen presentation. Most importantly, in which cellular compartments/vesicles the antigen is processed is critical to determine the type of immune response (cellular versus humoral).

The aim of this project is to characterize the conformational changes of the endocytic and secretory pathways taking place during dendritic cells activation, using a combination of advance imaging approaches, such as fluorescence microscopy, correlative light and electron microscopy, as well as high resolution Serial Blockface Scanning Electron Microscopy.
Impact of the project

How an antigen is processed and presented by dendritic cells is key for the development of specific (cellular or humoral) immunity. A better understanding of the changes in the endocytic and secretory pathways and how it differs with different antigens is key the key for vaccine development.



Faculty Research Themes

Infection and Immunology



Research Opportunities

Masters by Research, Honours, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.

Department

Biochemistry and Molecular Biology

Research Node

Bio21 Molecular Science and Biotechnology Institute

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