Cerebrospinal fluid biomarkers for aneurysmal subarachnoid haemorrhage

Research Opportunity
Honours students, Master of Biomedical Science
Number of Honour Places Available
Number of Master Places Available
Primary Supervisor Email Number Webpage
A/Prof James Ziogas jamesz@unimelb.edu.au

Summary Sub-arachnoid haemorrhage

Project Details

In the days following aneurysmal subarachnoid haemorrhage (aSAH) development of cerebral vasospasm (CVS) can lead to a general decrease in consciousness, delayed ischaemic neural deficits and cerebral infarction. The progression to a vasospastic state and its neurological sequelae represents an acutely debilitating pathology with a poor clinical prognosis and, for survivors, a high burden of disease (Rowland et al., 2012). Calcium channel antagonists such as nimodopine, which can ameliorate some of the vasoconstriction and excitotoxicity, are routinely given following surgical coiling or clipping of the aneurysm. However, further clinical intervention, currently hyperdynamic therapy or angioplasty, upon progression to a symptomatic vasospasm remains a necessity. In most cases, these interventions restore cerebral perfusion but have the potential for significant complications. Identification of appropriate biomarkers for the vasconstriction and neurological sequelae has the potential to inform improved post surgical management of aSAH.
Hypothesis: Development of CVS involves identifiable changes in the ratio of vasoactive, inflammatory and excitotoxic mediators following aSAH.
Specific aim: To obtain a temporal profile of functional, proteomic and metabolomic markers in cerebrospinal fluid (CSF) from patients following aSAH.
Nature of the work
The Department of Surgery at the Royal Melbourne Hospital (RMH) has 60-70 cases of aSAH per annum and collects CSF as part of the routine care of patients post- surgery. We have received approval from the RMH Human research ethics committee (MH Project number 2012.50) to undertake proteomic and metabolomic analysis of the CSF from these patients. Preliminary data indicate that ratiometric changes in certain proteins in the 10 – 40 kDa range may predict the likelihood of a patient developing CVS. This project will seek to extend these studies to include an analysis of proteins in higher and lower MW ranges (Rowland MJ, Hadjipavlou G, Klly M, Westbrook J & Pattison KTS. Delayed cerebral ischaemia after subarachnoid haemorrhage: looking beyond vasospasm. British Journal of Anaesthesia 109: 315-29).

Research Opportunities

Honours students, Master of Biomedical Science
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

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