Cell fates and cell states: analysis of enhancer dynamics during angiogenesis and lymphangiogenesis

Research Opportunity
PhD students, Honours students
Number of Honour Places Available
1
Primary Supervisor Email Number Webpage
Prof Ben Hogan ben.hogan@petermac.org
Co-supervisor Email Number Webpage
Dr Elizabeth Mason

Summary Cellular fates are regulated by key transcription factors during vascular development, angiogenesis and lymphangiogenesis. In recent decades, analysis of vascular cell fates, such as artery, vein and lymphatic fates, has uncovered key transcription factors and target enhancer elements that regulate tissue identity. Nevertheless, how transcription factors drive dynamic changes in vessel growth, dynamic enhancer activities, dynamic cell behaviours and cellular heterogeneity in the growing vasculature, remains to be determined. Live imaging reporters of enhancer activity during zebrafish vascular development offers a unique opportunity to approach these fundamental questions. This project will take advantage of a large-scale dataset recently generated in the Hogan lab using single cell ATAC-seq data to assess the developing vasculature of the zebrafish embryo. The project will clone and assess functional enhancers that are lineage specific, evolutionarily conserved and candidate elements that may control dynamic cell behaviours during new vessel formation. Transgenesis, molecular genetics and cellular resolution confocal imaging of zebrafish vasculature will be coupled with bioinformatics studies of enhancer conservation and prediction of key functional regulators.

Project Details

Cellular fates are regulated by key transcription factors
during vascular development, angiogenesis and
lymphangiogenesis. In recent decades, analysis of vascular
cell fates, such as artery, vein and lymphatic fates, has
uncovered key transcription factors and target enhancer
elements that regulate tissue identity. Nevertheless,
how transcription factors drive dynamic changes in
vessel growth, dynamic enhancer activities, dynamic cell
behaviours and cellular heterogeneity in the growing
vasculature, remains to be determined. Live imaging
reporters of enhancer activity during zebrafish vascular
development offers a unique opportunity to approach these
fundamental questions.
This project will take advantage of a large-scale dataset
recently generated in the Hogan lab using single cell
ATAC-seq data to assess the developing vasculature
of the zebrafish embryo. The project will clone and
assess functional enhancers that are lineage specific,
evolutionarily conserved and candidate elements that
may control dynamic cell behaviours during new vessel
formation. Transgenesis, molecular genetics and cellular
resolution confocal imaging of zebrafish vasculature
will be coupled with bioinformatics studies of enhancer
conservation and prediction of key functional regulators.



Research Opportunities

PhD students, Honours students
Students who are interested in joining this project will need to consider their elegibility as well as other requirements before contacting the supervisor of this research

Graduate Research application

Honours application

Key Contact

For further information about this research, please contact a supervisor.


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