An ultrasensitive assay to detect aggrecan degradation products in patient fluids
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Project Leader
Professor Amanda Fosang
Project Details
We are developing an ultrasensitive ELISA assay for detecting aggrecan degradation products in patient sera and synovial fluids. Using this assay, we will aim to quantitate aggrecan fragments that were previously at the limits of detection. We will be able to build a more detailed picture of how aggrecan is degraded in adult OA and juvenile arthritis.
Researchers
- Ms Suzanne Golub, Research Assistant
- Ms Karena Last, Research Assistant
- Ms Jia-Xi Han, Research Assistant
- Ms Lynette Ong, PhD Student
- Dr Heather Stanton, Research Officer/Administrator
Collaborators
- Professor Hideaki Nagase, Kazuhiro Yamamoto & Dr Linda Troeberg, Kennedy Institute of Rheumatology, Oxford, UK
- Dr Anne-Marie Malfait & Dr Rachel Miller, Rush University, Chicago, USA
- Prof Alan Grodzinsky, Massachusetts Institute of Technology, Boston, USA
- Prof Virginia Kraus, Duke University, USA
- Prof Anders Aspberg, University of Copenhagen, Denmark
- Prof Frank Beier, University of Western Ontario, Canada
- Dr Paul Holden, Oregon Health & Science University, Oregon, USA
- Prof Danny Chan, University of Hong Kong, China
- A/Prof Philip Sutton, MCRI
- Prof John Bateman, MCRI
- A/Prof Shireen Lamande, MCRI
- Dr Marc Seal, Royal Children’s Hospital
- Prof David Jackson, University of Melbourne
- Prof Eleanor Mackie, University of Melbourne
- A/Prof Natalie Sims, St Vincent’s Institute, Melbourne
- Prof Chris Little, University of Sydney, NSW
Funding
- USA Department of Defense
- National Health & Medical Research Council
- Australian Research Council
Research Outcomes
Struglics, A., Lohmander, S., Last K., Akikusa J., Allen R. & Fosang, AJ. (2012) Aggrecanase cleavage in juvenile idiopathic arthritis patients is minimally detected in the aggrecan interglobular domain but robust at the aggrecan C-terminus. Arthritis Rheum 64, 4151-4161.