To examine genetic variants of Nfkb1 as a biomarker of poor maternal health

Research Opportunity
PhD, Masters by Research, Honours, Master of Biomedical Science
Project Status
Future
Department
Medicine and Radiology
Location
Royal Melbourne Hospital
Supervisor Email Number Webpage
Dr Raffi Gugasyan Personal web page
Dr Philippe Boeuf Personal web page

Project Details

Healthy Mothers, Healthy Babies (HMHB) aims to define the major causes of poor maternal, newborn, and child health. Poor pregnancy outcomes, including anaemia, low birth weight, premature delivery and stillbirths are quite common. To identify feasible, acceptable and effective interventions it will be important to recognise those at highest risk.

The transcription factor Nuclear Factor-kappaB1 (NF-kB1) is an essential protein that regulates key physiological processes such as ageing, growth and immune competence. Insufficient production of NF-kB1 may lead to severe complications that become most prevalent during the fertile years of a woman’s life. Moreover, recent evidence suggests that genetic variants of NFKB1 alter protein levels that can affect idiopathic recurrent miscarriages.

This project will involve the genetic screening of NFKB1 variants to establish whether such variants correlate with the increased risk of poor pregnancy outcomes. The student will learn conventional PCR technology to screen for genetic variants of NFKB1 in 700 women from rural PNG. CRISPR/Cas9 will be used to examine these variants in cell lines and establish how they alter protein levels. We will determine whether variants of NFKB1 are a suitable biomarker for poor health and pregnancy outcomes, including miscarriages, which may facilitate early intervention and appropriate treatment regimens



Faculty Research Themes

Infection and Immunology

School Research Themes



Research Opportunities

PhD, Masters by Research, Honours, Master of Biomedical Science Graduate Research Students who are interested in joining this project will need to consider their elegibility as well as other Graduate Research requirements before contacting the supervisor of this research

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Key Contact

For further information about this research, please contact a supervisor.

Department

Medicine and Radiology

Research Group / Unit / Centre

Research Node

Royal Melbourne Hospital