Connecting a macro-vessel to a pre-vascularized scaffold

Research Opportunity
PhD, Masters by Research, Honours, Master of Biomedical Science
Project Status
Future
Department
Surgery
Location
St Vincent's Hospital
Supervisor Email Number Webpage
A/Prof Geraldine Mitchell Personal web page

Project Details

We have assembled pre-vascularized scaffolds in the laboratory, by seeding human primary microvascular endothelial cells into a porous scaffold, with an interconnected human capillary network assembling within 24 hours.    These pre-vascularized scaffolds could be used in wound healing or tissue engineering studies where organs are grown.    The implantation of pre-vascularized scaffolds in vivo, under the skin, would allow the human capillary network to functionally unite with host capillaries - this process is called inosculation and takes at least 2-3 days for host blood to flow through the scaffold capillaries.

An alternative approach where a blood flow would commence in a far shorter period throughout the scaffold, is possible if the pre-vascularized scaffold capillaries are connected via vascular connections to a large blood vessel.  We will investigate under culture conditions the connection of a large human blood vessel to a pre-vascularized scaffold.

We will also attempt to connect a pre-vascularized scaffold via vascular connections to a large blood vessel in an animal model. The project will involve cell culture, in vitro assembly of human capillary networks in scaffolds and vascular connection to a large blood vessel, and animal implantation studies where morphology/immunohistochemistry/ morphometry, and vascular perfusion techniques will be used to analyze the scaffolds.

This project is conducted in St Vincent’s Institute of Medical Research, Vascular Biology Group, (O’Brien Institute Department of SVI).


School Research Themes

Cardiometabolic



Research Opportunities

PhD, Masters by Research, Honours, Master of Biomedical Science
Graduate Research Students who are interested in joining this project will need to consider their elegibility as well as other Graduate Research requirements before contacting the supervisor of this research

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Key Contact

For further information about this research, please contact a supervisor.

Department

Surgery

Research Node

St Vincent's Hospital