Characterization of GPR35 in models of cardiovascular disease

Research Opportunity
Honours, Master of Biomedical Science
Project Status
Future
Department
Medicine and Radiology
Location
St Vincent's Hospital
Supervisor Email Number Webpage
Dr Andrew Kompa akompa@unimelb.edu.au Personal web page
Dr Michael Zhang Personal web page

Project Details

G-protein coupled receptor 35 (GPR 35) expression is increased early in the hearts animal models of cardiac disease such as myocardial infarction and models of hypertrophy, and in heart failure patients. Furthermore, in neonatal cardiomyocytes exposed to hypoxia, GPR 35 expression is increased after 12 hours and this increase is mediated by hypoxia-inducible factor 1 (HIF-1). A human polymorphism for this receptor has been identified and is associated with high coronary artery calcification, a measure of subclinical coronary atherosclerosis, which has been used to predict coronary artery disease events.  Evidence suggests that GPR35 may be an early marker of cardiac pathology and may also be a potential target for the development of novel therapies.

This project will assess the expression of GPR 35 in archived tissues from various animal models of cardiac disease, including myocardial infarction, pressure-overload hypertrophy and diabetes using immunohistochemistry and real time PCR. Under hypoxic conditions, known to increase GPR35, cell culture experiments utilising cardiac cells will be performed in the absence and presence of selective GPR 35 antagonists to determine the effect on GPR 35 gene expression, as well as determine effects on collagen synthesis and hypertrophy using proline and leucine incorporation assays respectively.
This project is suited for an Honours/Masters research project.


School Research Themes

Cardiometabolic



Research Opportunities

Honours, Master of Biomedical Science
Graduate Research Students who are interested in joining this project will need to consider their elegibility as well as other Graduate Research requirements before contacting the supervisor of this research

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Key Contact

For further information about this research, please contact a supervisor.

Department

Medicine and Radiology

Research Node

St Vincent's Hospital