Understanding how A-to-I RNA editing modifies the immunogenicity of endogenous RNA

Research Opportunity
PhD, Masters by Research, Honours, Master of Biomedical Science
Project Status
Future
Department
Medicine and Radiology
Location
St Vincent's Hospital
Supervisor Email Number Webpage
Dr Carl Walkley Personal web page
Dr Alistair Chalk Personal web page

Project Details

RNA editing, principally A-to-I editing, is the most prevalent form of RNA base modification and can lead to structural and functional changes in RNA and any subsequently encoded protein. Genomically encoded adenosine (A) is converted to inosine (I) in double stranded RNA (dsRNA) substrates. Inosine is interpreted as a guanine (G) during translation, thus harboring the potential to alter the protein coding sequence of mRNA substrates. However, A-to-I editing predominantly occurs in non-coding, repetitive elements such as inverted Alu elements and short interspersed elements (SINE). Estimates of the number of editing sites range from hundreds of thousands to millions in human cells, with tens of thousands in the mouse.

Our recent work has defined a close link between ADAR1, RNA editing and the innate immune system (see Hartner et al Nat Immunol 2009; Liddicoat et al, Science 2015; Liddicoat et al Exp Hematology 2016; Heraud-Farlow et al J Mol Med 2016; Heraud-Farlow et al Genome Biol 2017 in press). This project will apply unique mouse models to definitively understand the consequences of ADAR1 editing on non-coding and small RNA species. The project will involve both wet (tissue culture, primary cell models, RNA biology) and dry lab approaches (computational analysis, bioinformatics).

This project is conducted in St Vincent’s Institute of Medical Research, Stem Cell Regulation Unit.



Faculty Research Themes

Cancer, Infection and Immunology

School Research Themes

Cancer in Medicine



Research Opportunities

PhD, Masters by Research, Honours, Master of Biomedical Science Graduate Research Students who are interested in joining this project will need to consider their elegibility as well as other Graduate Research requirements before contacting the supervisor of this research

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Key Contact

For further information about this research, please contact a supervisor.

Department

Medicine and Radiology

Research Node

St Vincent's Hospital